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mouse igf ii duoset elisa kit  (R&D Systems)


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    R&D Systems mouse igf ii duoset elisa kit
    Figure 6. iTgMir483 mice show mild dyslipidemia, altered IGF1 and GH levels, and increased lipid production in the liver (A) Serum lipid biochemistry showing moderate dyslipidemia in W8 iTGMir483 adults compared to age-matched controls (FFA, free fatty acids; n = 6–13 per group). (B) Circulating plasma IGF1 levels are severely reduced in W15 iTGMir483 adults compared to age-matched controls (left, western blotting; right, quantification; n = 5 per group). (C) Circulating plasma GH levels are increased in W15 iTGMir483 adults compared to age-matched controls (measurements done by <t>ELISA</t> in n = 7–9 per group). (D) Livers are significantly lighter in W8 and W15 iTGMir483 adults compared to age-matched controls (n = 6–16 per group).
    Mouse Igf Ii Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igf+ii+duoset+elisa+kit/Mouse+IGF-II%2FIGF2+DuoSet+ELISA/pm39283743-849-10-15
    Average 92 stars, based on 15 article reviews
    mouse igf ii duoset elisa kit - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice."

    Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.114750

    Figure 6. iTgMir483 mice show mild dyslipidemia, altered IGF1 and GH levels, and increased lipid production in the liver (A) Serum lipid biochemistry showing moderate dyslipidemia in W8 iTGMir483 adults compared to age-matched controls (FFA, free fatty acids; n = 6–13 per group). (B) Circulating plasma IGF1 levels are severely reduced in W15 iTGMir483 adults compared to age-matched controls (left, western blotting; right, quantification; n = 5 per group). (C) Circulating plasma GH levels are increased in W15 iTGMir483 adults compared to age-matched controls (measurements done by ELISA in n = 7–9 per group). (D) Livers are significantly lighter in W8 and W15 iTGMir483 adults compared to age-matched controls (n = 6–16 per group).
    Figure Legend Snippet: Figure 6. iTgMir483 mice show mild dyslipidemia, altered IGF1 and GH levels, and increased lipid production in the liver (A) Serum lipid biochemistry showing moderate dyslipidemia in W8 iTGMir483 adults compared to age-matched controls (FFA, free fatty acids; n = 6–13 per group). (B) Circulating plasma IGF1 levels are severely reduced in W15 iTGMir483 adults compared to age-matched controls (left, western blotting; right, quantification; n = 5 per group). (C) Circulating plasma GH levels are increased in W15 iTGMir483 adults compared to age-matched controls (measurements done by ELISA in n = 7–9 per group). (D) Livers are significantly lighter in W8 and W15 iTGMir483 adults compared to age-matched controls (n = 6–16 per group).

    Techniques Used: Clinical Proteomics, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Uncoupling Exercise Bioenergetics From Systemic Metabolic Homeostasis by Conditional Inactivation of Baf60 in Skeletal Muscle.
    Article Snippet: .. Then, 200 mL reagent diluent was added to each sample to dissolve, from which 100 mL was used to measure insulin-like growth factor 2 (IGF2) levels using a Mouse IGF-II DuoSet ELISA Kit (R&D systems) according to the manufacturer’s protocol. ..

    Article Title: Mesenchymal Igf2 is a major paracrine regulator of pancreatic growth and function
    Article Snippet: .. Dry pellets were re-dissolved in 50 μl RIPA buffer and IGF2 measured by ELISA (Mouse IGF-II DuoSet ELISA kit, R&D Systems – DY792) using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as recently described . ..

    Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
    Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. Plasma IGF2 measurements IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Reagent or resource Source Identifier Antibodies Rat anti-mouse IGF-II R&D Systems RRID: AB_2122524 Biotinylated goat anti-mouse IGF-II R&D Systems RRID: AB_2122525 Anti-DIG-AP antibody Roche RRID: AB_2734716 Goat anti-human IGF2 R&D Systems RRID: AB_354449 Goat anti-mouse SOD1 R&D Systems RRID: AB_2239724 Rabbit anti-goat IgG-HRP Santa Cruz RRID: AB_656964 Goat anti-GFP Abcam RRID: AB_305643 Rabbit anti-CD31 Abcam RRID: AB_726362 Goat anti-CD31 R&D Systems RRID: AB_2161028 Rat anti-mouse F4/80 Bio-Rad RRID: AB_2098196 Chicken anti-MCT1 Merk Millipore RRID: AB_90565 Rabbit anti-MCT4 Merk Millipore RRID: AB_2286063 Rabbit anti-laminin Dako RRID: AB_2313665 Rabbit anti-pan cytokeratin Novus Biologicals RRID: AB_2296857 Rat anti-mouse CD326/Epcam BD Biosciences RRID: AB_394370 Donkey anti-goat-AF488 Jackson ImmunoResearch RRID: AB_2340430 Donkey anti-rabbit-AF594 Jackson ImmunoResearch RRID: AB_2340619 Goat anti-rabbit, biotinylated Abcam RRID: AB_954902 NL557-conjugated donkey anti-goat R&D Systems RRID: AB_663766) Rabbit anti-rat Bethyl RRID: AB_10681533 Anti-rabbit HRP Vector Labs RRID: AB_2631198 Donkey anti-chicken-AF488 Jackson ImmunoResearch RRID: AB_2340376 Goat anti-rabbit-AP Abcam RRID: AB_954595 Donkey anti-rat-AF594 Thermo Fisher Scientific RRID: AB_2535795 Rat anti-CD16/32 BioLegend RRID: AB_1574975 Rat anti-mouse CD41-PE BioLegend RRID: AB_2129745 Rat anti-mouse CD31-AF647 BioLegend RRID: AB_2161029 Rat anti-mouse CD326-AF647 BioLegend RRID: AB_1134101 Rat anti-mouse CD41-BV421 BioLegend RRID: AB_10960744 Rat anti-mouse CD117-PE BioLegend RRID: AB_313217 Rat anti-mouse Ly-6A/E-BV510 BioLegend RRID: AB_2561593 Rat anti-lineage cocktail-BV421 BioLegend RRID: AB_11203535 Rat anti-mouse CD45-V500 BD Biosciences RRID: AB_10697046 Rat anti-mouse CD34-AF700 Thermo Fisher Scientific RRID: AB_493998 Rabbit anti IGF2R Cell Signaling RRID: AB_2798462 Mouse anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling RRID: AB_331768 Rabbit anti-p44/42 MAPK (Erk1/2) Cell Signaling RRID: AB_330744 Rabbit anti-Phospho-Akt (Ser473) Cell Signaling RRID: AB_329825 Rabbit anti-Akt Cell Signaling RRID: AB_329827 Rabbit anti-HSP90 Cell Signaling RRID: AB_2233307 Chemicals, peptides, and recombinant proteins RIPA buffer Sigma R0278 (Continued on next page) e1 Developmental Cell 57, 63–79.e1–e8, January 10, 2022

    Article Title: The imprinted Mir483 is a growth suppressor and metabolic regulator functioning through IGF1
    Article Snippet: .. IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as previously described . ..

    Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
    Article Snippet: .. IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as previously described78. ..

    Article Title: Uncoupling Exercise Bioenergetics From Systemic Metabolic Homeostasis by Conditional Inactivation of Baf60 in Skeletal Muscle
    Article Snippet: .. Then, 200 μL reagent diluent was added to each sample to dissolve, from which 100 μL was used to measure insulin-like growth factor 2 (IGF2) levels using a Mouse IGF-II DuoSet ELISA Kit (R&D systems) according to the manufacturer's protocol. ..

    Article Title: The Imprinted Igf2 - Igf2r Axis is Critical for Matching Placental Microvasculature Expansion to Fetal Growth
    Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.

    Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
    Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in ), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.

    Electrochemiluminescence:

    Article Title: Mesenchymal Igf2 is a major paracrine regulator of pancreatic growth and function
    Article Snippet: .. Dry pellets were re-dissolved in 50 μl RIPA buffer and IGF2 measured by ELISA (Mouse IGF-II DuoSet ELISA kit, R&D Systems – DY792) using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as recently described . ..

    Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
    Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. Plasma IGF2 measurements IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Reagent or resource Source Identifier Antibodies Rat anti-mouse IGF-II R&D Systems RRID: AB_2122524 Biotinylated goat anti-mouse IGF-II R&D Systems RRID: AB_2122525 Anti-DIG-AP antibody Roche RRID: AB_2734716 Goat anti-human IGF2 R&D Systems RRID: AB_354449 Goat anti-mouse SOD1 R&D Systems RRID: AB_2239724 Rabbit anti-goat IgG-HRP Santa Cruz RRID: AB_656964 Goat anti-GFP Abcam RRID: AB_305643 Rabbit anti-CD31 Abcam RRID: AB_726362 Goat anti-CD31 R&D Systems RRID: AB_2161028 Rat anti-mouse F4/80 Bio-Rad RRID: AB_2098196 Chicken anti-MCT1 Merk Millipore RRID: AB_90565 Rabbit anti-MCT4 Merk Millipore RRID: AB_2286063 Rabbit anti-laminin Dako RRID: AB_2313665 Rabbit anti-pan cytokeratin Novus Biologicals RRID: AB_2296857 Rat anti-mouse CD326/Epcam BD Biosciences RRID: AB_394370 Donkey anti-goat-AF488 Jackson ImmunoResearch RRID: AB_2340430 Donkey anti-rabbit-AF594 Jackson ImmunoResearch RRID: AB_2340619 Goat anti-rabbit, biotinylated Abcam RRID: AB_954902 NL557-conjugated donkey anti-goat R&D Systems RRID: AB_663766) Rabbit anti-rat Bethyl RRID: AB_10681533 Anti-rabbit HRP Vector Labs RRID: AB_2631198 Donkey anti-chicken-AF488 Jackson ImmunoResearch RRID: AB_2340376 Goat anti-rabbit-AP Abcam RRID: AB_954595 Donkey anti-rat-AF594 Thermo Fisher Scientific RRID: AB_2535795 Rat anti-CD16/32 BioLegend RRID: AB_1574975 Rat anti-mouse CD41-PE BioLegend RRID: AB_2129745 Rat anti-mouse CD31-AF647 BioLegend RRID: AB_2161029 Rat anti-mouse CD326-AF647 BioLegend RRID: AB_1134101 Rat anti-mouse CD41-BV421 BioLegend RRID: AB_10960744 Rat anti-mouse CD117-PE BioLegend RRID: AB_313217 Rat anti-mouse Ly-6A/E-BV510 BioLegend RRID: AB_2561593 Rat anti-lineage cocktail-BV421 BioLegend RRID: AB_11203535 Rat anti-mouse CD45-V500 BD Biosciences RRID: AB_10697046 Rat anti-mouse CD34-AF700 Thermo Fisher Scientific RRID: AB_493998 Rabbit anti IGF2R Cell Signaling RRID: AB_2798462 Mouse anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling RRID: AB_331768 Rabbit anti-p44/42 MAPK (Erk1/2) Cell Signaling RRID: AB_330744 Rabbit anti-Phospho-Akt (Ser473) Cell Signaling RRID: AB_329825 Rabbit anti-Akt Cell Signaling RRID: AB_329827 Rabbit anti-HSP90 Cell Signaling RRID: AB_2233307 Chemicals, peptides, and recombinant proteins RIPA buffer Sigma R0278 (Continued on next page) e1 Developmental Cell 57, 63–79.e1–e8, January 10, 2022

    Article Title: The imprinted Mir483 is a growth suppressor and metabolic regulator functioning through IGF1
    Article Snippet: .. IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as previously described . ..

    Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.
    Article Snippet: .. IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as previously described78. ..

    Article Title: The Imprinted Igf2 - Igf2r Axis is Critical for Matching Placental Microvasculature Expansion to Fetal Growth
    Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.

    Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
    Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in ), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.

    Clinical Proteomics:

    Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
    Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. Plasma IGF2 measurements IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Reagent or resource Source Identifier Antibodies Rat anti-mouse IGF-II R&D Systems RRID: AB_2122524 Biotinylated goat anti-mouse IGF-II R&D Systems RRID: AB_2122525 Anti-DIG-AP antibody Roche RRID: AB_2734716 Goat anti-human IGF2 R&D Systems RRID: AB_354449 Goat anti-mouse SOD1 R&D Systems RRID: AB_2239724 Rabbit anti-goat IgG-HRP Santa Cruz RRID: AB_656964 Goat anti-GFP Abcam RRID: AB_305643 Rabbit anti-CD31 Abcam RRID: AB_726362 Goat anti-CD31 R&D Systems RRID: AB_2161028 Rat anti-mouse F4/80 Bio-Rad RRID: AB_2098196 Chicken anti-MCT1 Merk Millipore RRID: AB_90565 Rabbit anti-MCT4 Merk Millipore RRID: AB_2286063 Rabbit anti-laminin Dako RRID: AB_2313665 Rabbit anti-pan cytokeratin Novus Biologicals RRID: AB_2296857 Rat anti-mouse CD326/Epcam BD Biosciences RRID: AB_394370 Donkey anti-goat-AF488 Jackson ImmunoResearch RRID: AB_2340430 Donkey anti-rabbit-AF594 Jackson ImmunoResearch RRID: AB_2340619 Goat anti-rabbit, biotinylated Abcam RRID: AB_954902 NL557-conjugated donkey anti-goat R&D Systems RRID: AB_663766) Rabbit anti-rat Bethyl RRID: AB_10681533 Anti-rabbit HRP Vector Labs RRID: AB_2631198 Donkey anti-chicken-AF488 Jackson ImmunoResearch RRID: AB_2340376 Goat anti-rabbit-AP Abcam RRID: AB_954595 Donkey anti-rat-AF594 Thermo Fisher Scientific RRID: AB_2535795 Rat anti-CD16/32 BioLegend RRID: AB_1574975 Rat anti-mouse CD41-PE BioLegend RRID: AB_2129745 Rat anti-mouse CD31-AF647 BioLegend RRID: AB_2161029 Rat anti-mouse CD326-AF647 BioLegend RRID: AB_1134101 Rat anti-mouse CD41-BV421 BioLegend RRID: AB_10960744 Rat anti-mouse CD117-PE BioLegend RRID: AB_313217 Rat anti-mouse Ly-6A/E-BV510 BioLegend RRID: AB_2561593 Rat anti-lineage cocktail-BV421 BioLegend RRID: AB_11203535 Rat anti-mouse CD45-V500 BD Biosciences RRID: AB_10697046 Rat anti-mouse CD34-AF700 Thermo Fisher Scientific RRID: AB_493998 Rabbit anti IGF2R Cell Signaling RRID: AB_2798462 Mouse anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling RRID: AB_331768 Rabbit anti-p44/42 MAPK (Erk1/2) Cell Signaling RRID: AB_330744 Rabbit anti-Phospho-Akt (Ser473) Cell Signaling RRID: AB_329825 Rabbit anti-Akt Cell Signaling RRID: AB_329827 Rabbit anti-HSP90 Cell Signaling RRID: AB_2233307 Chemicals, peptides, and recombinant proteins RIPA buffer Sigma R0278 (Continued on next page) e1 Developmental Cell 57, 63–79.e1–e8, January 10, 2022



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    Figure 6. iTgMir483 mice show mild dyslipidemia, altered IGF1 and GH levels, and increased lipid production in the liver (A) Serum lipid biochemistry showing moderate dyslipidemia in W8 iTGMir483 adults compared to age-matched controls (FFA, free fatty acids; n = 6–13 per group). (B) Circulating plasma IGF1 levels are severely reduced in W15 iTGMir483 adults compared to age-matched controls (left, western blotting; right, quantification; n = 5 per group). (C) Circulating plasma GH levels are increased in W15 iTGMir483 adults compared to age-matched controls (measurements done by <t>ELISA</t> in n = 7–9 per group). (D) Livers are significantly lighter in W8 and W15 iTGMir483 adults compared to age-matched controls (n = 6–16 per group).
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    FIGURE 5 qPCR for Tnf (A), Igf1 (B), Egfr (C), <t>Igf2</t> (D), and Egf mRNA (E) and <t>ELISA</t> for IGF2 (F) in mammary glands on PND 42 from females exposed to (via dam nutrition), and then fed, control or folic acid–deficient diets (study 1). Values are means ± SDs. (A–E) n = 3, (F) n = 8. Labeled means without a common letter differ, P < 0.05. Egf, epidermal growth factor; Egfr, epidermal growth factor receptor; Igf1, insulin-like growth factor 1; Igf2, insulin-like growth factor 2.
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    Image Search Results


    Figure 6. iTgMir483 mice show mild dyslipidemia, altered IGF1 and GH levels, and increased lipid production in the liver (A) Serum lipid biochemistry showing moderate dyslipidemia in W8 iTGMir483 adults compared to age-matched controls (FFA, free fatty acids; n = 6–13 per group). (B) Circulating plasma IGF1 levels are severely reduced in W15 iTGMir483 adults compared to age-matched controls (left, western blotting; right, quantification; n = 5 per group). (C) Circulating plasma GH levels are increased in W15 iTGMir483 adults compared to age-matched controls (measurements done by ELISA in n = 7–9 per group). (D) Livers are significantly lighter in W8 and W15 iTGMir483 adults compared to age-matched controls (n = 6–16 per group).

    Journal: Cell reports

    Article Title: Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice.

    doi: 10.1016/j.celrep.2024.114750

    Figure Lengend Snippet: Figure 6. iTgMir483 mice show mild dyslipidemia, altered IGF1 and GH levels, and increased lipid production in the liver (A) Serum lipid biochemistry showing moderate dyslipidemia in W8 iTGMir483 adults compared to age-matched controls (FFA, free fatty acids; n = 6–13 per group). (B) Circulating plasma IGF1 levels are severely reduced in W15 iTGMir483 adults compared to age-matched controls (left, western blotting; right, quantification; n = 5 per group). (C) Circulating plasma GH levels are increased in W15 iTGMir483 adults compared to age-matched controls (measurements done by ELISA in n = 7–9 per group). (D) Livers are significantly lighter in W8 and W15 iTGMir483 adults compared to age-matched controls (n = 6–16 per group).

    Article Snippet: IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as previously described78.

    Techniques: Clinical Proteomics, Western Blot, Enzyme-linked Immunosorbent Assay

    FIGURE 5 qPCR for Tnf (A), Igf1 (B), Egfr (C), Igf2 (D), and Egf mRNA (E) and ELISA for IGF2 (F) in mammary glands on PND 42 from females exposed to (via dam nutrition), and then fed, control or folic acid–deficient diets (study 1). Values are means ± SDs. (A–E) n = 3, (F) n = 8. Labeled means without a common letter differ, P < 0.05. Egf, epidermal growth factor; Egfr, epidermal growth factor receptor; Igf1, insulin-like growth factor 1; Igf2, insulin-like growth factor 2.

    Journal: The Journal of nutrition

    Article Title: Folate Deficiency Inhibits Development of the Mammary Gland and its Associated Lymphatics in FVB Mice.

    doi: 10.1093/jn/nxaa154

    Figure Lengend Snippet: FIGURE 5 qPCR for Tnf (A), Igf1 (B), Egfr (C), Igf2 (D), and Egf mRNA (E) and ELISA for IGF2 (F) in mammary glands on PND 42 from females exposed to (via dam nutrition), and then fed, control or folic acid–deficient diets (study 1). Values are means ± SDs. (A–E) n = 3, (F) n = 8. Labeled means without a common letter differ, P < 0.05. Egf, epidermal growth factor; Egfr, epidermal growth factor receptor; Igf1, insulin-like growth factor 1; Igf2, insulin-like growth factor 2.

    Article Snippet: Homogenates were assayed for mouse IGF2 in triplicate using the IGF2 DuoSet ELISA kit and DuoSet ELISA ancillary reagent kit 3 (R&D Systems Inc, Minnesota) against IGF2 standard curves prepared in homogenization buffer.

    Techniques: Enzyme-linked Immunosorbent Assay, Control, Labeling