Enzyme-linked Immunosorbent Assay:
Article Title: Mesenchymal Igf2 is a major paracrine regulator of pancreatic growth and function
Article Snippet: .. Dry pellets were re-dissolved in 50 μl RIPA buffer and IGF2 measured by ELISA (Mouse IGF-II DuoSet ELISA kit, R&D Systems – DY792) using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as recently described . ..
Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. Plasma IGF2 measurements IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Reagent or resource Source Identifier Antibodies Rat anti-mouse IGF-II R&D Systems RRID: AB_2122524 Biotinylated goat anti-mouse IGF-II R&D Systems RRID: AB_2122525 Anti-DIG-AP antibody Roche RRID: AB_2734716 Goat anti-human IGF2 R&D Systems RRID: AB_354449 Goat anti-mouse SOD1 R&D Systems RRID: AB_2239724 Rabbit anti-goat IgG-HRP Santa Cruz RRID: AB_656964 Goat anti-GFP Abcam RRID: AB_305643 Rabbit anti-CD31 Abcam RRID: AB_726362 Goat anti-CD31 R&D Systems RRID: AB_2161028 Rat anti-mouse F4/80 Bio-Rad RRID: AB_2098196 Chicken anti-MCT1 Merk Millipore RRID: AB_90565 Rabbit anti-MCT4 Merk Millipore RRID: AB_2286063 Rabbit anti-laminin Dako RRID: AB_2313665 Rabbit anti-pan cytokeratin Novus Biologicals RRID: AB_2296857 Rat anti-mouse CD326/Epcam BD Biosciences RRID: AB_394370 Donkey anti-goat-AF488 Jackson ImmunoResearch RRID: AB_2340430 Donkey anti-rabbit-AF594 Jackson ImmunoResearch RRID: AB_2340619 Goat anti-rabbit, biotinylated Abcam RRID: AB_954902 NL557-conjugated donkey anti-goat R&D Systems RRID: AB_663766) Rabbit anti-rat Bethyl RRID: AB_10681533 Anti-rabbit HRP Vector Labs RRID: AB_2631198 Donkey anti-chicken-AF488 Jackson ImmunoResearch RRID: AB_2340376 Goat anti-rabbit-AP Abcam RRID: AB_954595 Donkey anti-rat-AF594 Thermo Fisher Scientific RRID: AB_2535795 Rat anti-CD16/32 BioLegend RRID: AB_1574975 Rat anti-mouse CD41-PE BioLegend RRID: AB_2129745 Rat anti-mouse CD31-AF647 BioLegend RRID: AB_2161029 Rat anti-mouse CD326-AF647 BioLegend RRID: AB_1134101 Rat anti-mouse CD41-BV421 BioLegend RRID: AB_10960744 Rat anti-mouse CD117-PE BioLegend RRID: AB_313217 Rat anti-mouse Ly-6A/E-BV510 BioLegend RRID: AB_2561593 Rat anti-lineage cocktail-BV421 BioLegend RRID: AB_11203535 Rat anti-mouse CD45-V500 BD Biosciences RRID: AB_10697046 Rat anti-mouse CD34-AF700 Thermo Fisher Scientific RRID: AB_493998 Rabbit anti IGF2R Cell Signaling RRID: AB_2798462 Mouse anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling RRID: AB_331768 Rabbit anti-p44/42 MAPK (Erk1/2) Cell Signaling RRID: AB_330744 Rabbit anti-Phospho-Akt (Ser473) Cell Signaling RRID: AB_329825 Rabbit anti-Akt Cell Signaling RRID: AB_329827 Rabbit anti-HSP90 Cell Signaling RRID: AB_2233307 Chemicals, peptides, and recombinant proteins RIPA buffer Sigma R0278 (Continued on next page) e1 Developmental Cell 57, 63–79.e1–e8, January 10, 2022
Article Title: The imprinted Mir483 is a growth suppressor and metabolic regulator functioning through IGF1
Article Snippet: .. IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as previously described . ..
Article Title: The Imprinted Igf2 - Igf2r Axis is Critical for Matching Placental Microvasculature Expansion to Fetal Growth
Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.
Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in ), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.
Electrochemiluminescence:Article Title: Mesenchymal Igf2 is a major paracrine regulator of pancreatic growth and function
Article Snippet: .. Dry pellets were re-dissolved in 50 μl RIPA buffer and IGF2 measured by ELISA (Mouse IGF-II DuoSet ELISA kit, R&D Systems – DY792) using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as recently described . ..
Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. Plasma IGF2 measurements IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Reagent or resource Source Identifier Antibodies Rat anti-mouse IGF-II R&D Systems RRID: AB_2122524 Biotinylated goat anti-mouse IGF-II R&D Systems RRID: AB_2122525 Anti-DIG-AP antibody Roche RRID: AB_2734716 Goat anti-human IGF2 R&D Systems RRID: AB_354449 Goat anti-mouse SOD1 R&D Systems RRID: AB_2239724 Rabbit anti-goat IgG-HRP Santa Cruz RRID: AB_656964 Goat anti-GFP Abcam RRID: AB_305643 Rabbit anti-CD31 Abcam RRID: AB_726362 Goat anti-CD31 R&D Systems RRID: AB_2161028 Rat anti-mouse F4/80 Bio-Rad RRID: AB_2098196 Chicken anti-MCT1 Merk Millipore RRID: AB_90565 Rabbit anti-MCT4 Merk Millipore RRID: AB_2286063 Rabbit anti-laminin Dako RRID: AB_2313665 Rabbit anti-pan cytokeratin Novus Biologicals RRID: AB_2296857 Rat anti-mouse CD326/Epcam BD Biosciences RRID: AB_394370 Donkey anti-goat-AF488 Jackson ImmunoResearch RRID: AB_2340430 Donkey anti-rabbit-AF594 Jackson ImmunoResearch RRID: AB_2340619 Goat anti-rabbit, biotinylated Abcam RRID: AB_954902 NL557-conjugated donkey anti-goat R&D Systems RRID: AB_663766) Rabbit anti-rat Bethyl RRID: AB_10681533 Anti-rabbit HRP Vector Labs RRID: AB_2631198 Donkey anti-chicken-AF488 Jackson ImmunoResearch RRID: AB_2340376 Goat anti-rabbit-AP Abcam RRID: AB_954595 Donkey anti-rat-AF594 Thermo Fisher Scientific RRID: AB_2535795 Rat anti-CD16/32 BioLegend RRID: AB_1574975 Rat anti-mouse CD41-PE BioLegend RRID: AB_2129745 Rat anti-mouse CD31-AF647 BioLegend RRID: AB_2161029 Rat anti-mouse CD326-AF647 BioLegend RRID: AB_1134101 Rat anti-mouse CD41-BV421 BioLegend RRID: AB_10960744 Rat anti-mouse CD117-PE BioLegend RRID: AB_313217 Rat anti-mouse Ly-6A/E-BV510 BioLegend RRID: AB_2561593 Rat anti-lineage cocktail-BV421 BioLegend RRID: AB_11203535 Rat anti-mouse CD45-V500 BD Biosciences RRID: AB_10697046 Rat anti-mouse CD34-AF700 Thermo Fisher Scientific RRID: AB_493998 Rabbit anti IGF2R Cell Signaling RRID: AB_2798462 Mouse anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling RRID: AB_331768 Rabbit anti-p44/42 MAPK (Erk1/2) Cell Signaling RRID: AB_330744 Rabbit anti-Phospho-Akt (Ser473) Cell Signaling RRID: AB_329825 Rabbit anti-Akt Cell Signaling RRID: AB_329827 Rabbit anti-HSP90 Cell Signaling RRID: AB_2233307 Chemicals, peptides, and recombinant proteins RIPA buffer Sigma R0278 (Continued on next page) e1 Developmental Cell 57, 63–79.e1–e8, January 10, 2022
Article Title: The imprinted Mir483 is a growth suppressor and metabolic regulator functioning through IGF1
Article Snippet: .. IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD), as previously described . ..
Article Title: The Imprinted Igf2 - Igf2r Axis is Critical for Matching Placental Microvasculature Expansion to Fetal Growth
Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.
Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in ), followed by separation of PCR amplicons by agarose gel electrophoresis. .. IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Briefly, MSD standard-bind microtitre plates were first coated with 30μl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 μg/ml in PBS, sealed, and incubated overnight at 4°C.
Clinical Proteomics:Article Title: The imprinted Igf2 - Igf2r axis is critical for matching placental microvasculature expansion to fetal growth
Article Snippet: PCR was performed using the Red Taq Ready PCR system (Sigma) (see list of primers in Table S6), followed by separation of PCR amplicons by agarose gel electrophoresis. .. Plasma IGF2 measurements IGF2 measurements were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD). .. Reagent or resource Source Identifier Antibodies Rat anti-mouse IGF-II R&D Systems RRID: AB_2122524 Biotinylated goat anti-mouse IGF-II R&D Systems RRID: AB_2122525 Anti-DIG-AP antibody Roche RRID: AB_2734716 Goat anti-human IGF2 R&D Systems RRID: AB_354449 Goat anti-mouse SOD1 R&D Systems RRID: AB_2239724 Rabbit anti-goat IgG-HRP Santa Cruz RRID: AB_656964 Goat anti-GFP Abcam RRID: AB_305643 Rabbit anti-CD31 Abcam RRID: AB_726362 Goat anti-CD31 R&D Systems RRID: AB_2161028 Rat anti-mouse F4/80 Bio-Rad RRID: AB_2098196 Chicken anti-MCT1 Merk Millipore RRID: AB_90565 Rabbit anti-MCT4 Merk Millipore RRID: AB_2286063 Rabbit anti-laminin Dako RRID: AB_2313665 Rabbit anti-pan cytokeratin Novus Biologicals RRID: AB_2296857 Rat anti-mouse CD326/Epcam BD Biosciences RRID: AB_394370 Donkey anti-goat-AF488 Jackson ImmunoResearch RRID: AB_2340430 Donkey anti-rabbit-AF594 Jackson ImmunoResearch RRID: AB_2340619 Goat anti-rabbit, biotinylated Abcam RRID: AB_954902 NL557-conjugated donkey anti-goat R&D Systems RRID: AB_663766) Rabbit anti-rat Bethyl RRID: AB_10681533 Anti-rabbit HRP Vector Labs RRID: AB_2631198 Donkey anti-chicken-AF488 Jackson ImmunoResearch RRID: AB_2340376 Goat anti-rabbit-AP Abcam RRID: AB_954595 Donkey anti-rat-AF594 Thermo Fisher Scientific RRID: AB_2535795 Rat anti-CD16/32 BioLegend RRID: AB_1574975 Rat anti-mouse CD41-PE BioLegend RRID: AB_2129745 Rat anti-mouse CD31-AF647 BioLegend RRID: AB_2161029 Rat anti-mouse CD326-AF647 BioLegend RRID: AB_1134101 Rat anti-mouse CD41-BV421 BioLegend RRID: AB_10960744 Rat anti-mouse CD117-PE BioLegend RRID: AB_313217 Rat anti-mouse Ly-6A/E-BV510 BioLegend RRID: AB_2561593 Rat anti-lineage cocktail-BV421 BioLegend RRID: AB_11203535 Rat anti-mouse CD45-V500 BD Biosciences RRID: AB_10697046 Rat anti-mouse CD34-AF700 Thermo Fisher Scientific RRID: AB_493998 Rabbit anti IGF2R Cell Signaling RRID: AB_2798462 Mouse anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Cell Signaling RRID: AB_331768 Rabbit anti-p44/42 MAPK (Erk1/2) Cell Signaling RRID: AB_330744 Rabbit anti-Phospho-Akt (Ser473) Cell Signaling RRID: AB_329825 Rabbit anti-Akt Cell Signaling RRID: AB_329827 Rabbit anti-HSP90 Cell Signaling RRID: AB_2233307 Chemicals, peptides, and recombinant proteins RIPA buffer Sigma R0278 (Continued on next page) e1 Developmental Cell 57, 63–79.e1–e8, January 10, 2022
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